INFECTION-REDUCTION RATES IN AMPICILLIN-RESISTANT Escherichia coli-INFECTED CHICKS TREATED WITH A FORMULATION OF AMPICILLIN-MEDICINAL SYNTHETIC ALUMINUM MAGNESIUM SILICATE (NANO-STABILIZING AGENT)

Abstract

Antimicrobial resistance (AMR) is a global concern that is threatening livestock production. In poultry, indiscriminate use of antibiotics has led to resistant strains of Escherichia coli, impairing treatment outcomes. Aluminum-magnesium silicate (AMS) is an approved nano-stabilizing agent. Stabilizing antimicrobials prolongs time they remain at high bioavailability. Nanoparticles also improve delivery of medicines to effect-targets. Prolonging medicines` time of high bioavailability and enhancing their delivery improve efficacy. Again, antioxidants improve hosts` immune functions. Enhanced efficacy allows lower dosages to achieve therapeutic effects, thereby minimizing immune suppression from medicines. Improving efficacy of medicines and immune response of patients could overcome AMR. Chicks were screened and assigned to five groups. Four were orally infected with 0.1 mL of E. coli (15×10⁶ CFU/mL), and three of these received ampicillin-MSAMS treatment for five days. One group received additional vitamin C support. Bile samples were cultured for colony-forming units (CFU), and reproductive tissues were examined histologically. Mean colony-forming units (CFU) of an ampicillin resistant E. coli infection in bile of chicks was 37.52 ± 3.13 per ml. At its 100 % dosage, Ampicillin reduced (P ≤ 0.05) the CFU to 9.80 ± 2.83 (74 %-reduction) while at 75 %-dosage, stabilizing the medicine with Medicinal synthetic AMS reduced (P ≤ 0.01) the CFU to 6.07 ± 0.30 (83 %-reduction,). Supporting the 75 % ampicillin-MSAMS dosage with Vitamin C reduced the CFU to 4.20 ± 0.15 (89 %-reduction). The results suggest that stabilizing Ampicillin`s 75 %-dosage with MSAMS and supporting the treatment with antioxidants restore its efficacy against AMR. We recommend incorporating nano-stabilizers and immune support in antimicrobial therapies to reduce dosage, enhance outcomes, and mitigate resistance development in poultry.